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The method of observation (Wang Zhen) follows specific principles, involving the differentiation of the Five Viscera (Zang Organs) and the distinction of the Six Regions (Liu Bu). The term "Six Regions" refers to the central areas housing the viscera as "internal," and the peripheral areas of limbs and joints as "external"; horizontally divided into upper and lower sections, and vertically divided into left and right sections. Regarding internal and external: if the complexion appears in the internal regions, the disease is internal; if it appears in the external regions, the disease is external. If the color moves from the external to the internal region, the pathogen moves from the exterior to the interior; if it moves from the internal to the external region, the pathogen moves from the interior to the exterior. Disease located internally is severe, while disease located externally is mild. Transmission from internal to external indicates the transmission of Zang disease to Fu organs, and Fu disease to the surface, representing a shift from severe to mild. Transmission from external to internal indicates the transmission of surface disease to Fu organs, and Fu disease to Zang organs, representing a shift from mild to severe. This constitutes the distinction between internal and external.

Regarding upper and lower: if the complexion appears in the upper section, the disease is in the upper part; if it appears in the lower section, the disease is in the lower part. If the color moves from the lower to the upper section, the disease progresses upward; if it moves from the upper to the lower section, the disease progresses downward. The upper belongs to Yang, and the lower belongs to Yin. In Yang diseases, progression reaches its extreme before descending; in Yin diseases, progression reaches its extreme before ascending. For Yang diseases, movement from upper to lower is considered severe, while movement from lower to upper is mild. For Yin diseases, movement from lower to upper is considered severe, while movement from upper to lower is mild. This constitutes the distinction between upper and lower.

Regarding left and right: if the complexion appears on the left side, the disease is on the left; if it appears on the right side, the disease is on the right. If the color moves from the right to the left section, the disease moves from right to left; if it moves from the left to the right section, the disease moves from left to right. The left belongs to Yang, and the right belongs to Yin. For men, the left side represents "Ni" (adverse/unfavorable), and the right side represents "Cong" (favorable/normal); for women, the right side represents "Ni," and the left side represents "Cong." For men, disease transmission from left to right is favorable, while from right to left is adverse; for women, disease transmission from right to left is favorable, while from left to right is adverse. This constitutes the distinction between left and right.

From this, when the Five Colors (Wu Se) intermingle and the Six Regions are analyzed together, there are respective directions of transmission and patterns of favorability or adversity. One must observe the origin of the color changes, distinguish the direction of their movement, thereby deducing the顺逆 (shun ni - favorable/adverse nature of the disease progression), and judge the auspiciousness or inauspiciousness. However, what is the rationale behind such movement of colors? Generally, if the color shape is sharp at the top, its movement is directed upward; if sharp at the bottom, its movement is directed downward. The same applies to the left and right sides, and similarly to the internal and external regions. This is the outline of the Six Regions. Qi Bo stated: "The viscera reside within, each occupying its designated position according to sequence; their arrangement on the left, right, upper, and lower corresponds precisely to these measures." This is exactly what is meant.